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staining with cd137  (Miltenyi Biotec)


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    Miltenyi Biotec staining with cd137
    CAR-T cells targeting the MICB-derived octapeptide show CAR-dependent activation and efficiently kill tumor cells (A) Schematic representation of scFv of both 8-VHVL CAR and 8-VLVH CAR targeting the octapeptide VLQSQRTD used in this study. (B) Expression of CAR molecules on T cells. Left panel shows representative plots of flow cytometry data of primary T cells, 8-VHVL CAR-T and 8-VLVH CAR-T. Right panel shows expression of both experimental CARs (8-VHVL and 8-VLVH) as well as CD4 and CD19 CAR on T cells. Black bars represent CAR expression detected via anti-mouse F(abʹ) 2 antibody, and green bars represent EGFP signal; data from n = 18 independent experiments are depicted as mean (SEM). (C) CAR-T cells were cocultured with Mac-1 cells stably transduced to express indicated transgenes. Tumor cells were labeled with CellTrace Violet, and killing was assessed via PI staining. Killing is depicted as ratio of PI+ cells in cocultures and independently cultured Mac-1 cells. Data from n ≥ 6 experiments are depicted as mean (SEM). (D) <t>CD137</t> expression was measured on CAR-T cells after incubation with Mac-1 for 24 h. Data are shown from EGFP+ CAR-T cells and are depicted from n = 6 independent experiments as mean (SEM). (E) CAR-T cells were cocultured with T cells from the same donor transduced to express indicated transgenes. T cells used as target cells were labeled with CellTrace Violet, and killing was assessed via PI staining. Only EGFP+ target T cells were analyzed for PI staining. Killing is depicted as the ratio of PI+ cells in cocultures and independently cultured target T cells. Data from n = 9 independent experiments are shown as mean (SEM). (F) CD137 expression was measured on CAR-T cells after incubation with transduced T cells as target cells for 24 h. Data are shown from EGFP+ CAR-T cells and are depicted from n = 6 independent experiments as mean (SEM). (G) LAG-3 expression was measured on CAR-T cells after incubation with transduced T cells as target cells for 72 h. Data are shown from EGFP+ CAR-T cells from n = 4 experiments as mean (SEM); statistical analysis of (C) through (G) was performed by two-way ANOVA followed by Dunnett’s multiple-comparisons test, and experimental groups were compared to CD19 CAR. ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001, and ∗∗∗∗ = p ≤ 0.0001.
    Staining With Cd137, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+with+anti+cd137/CD137+Antibody%2C+anti-human%2C+PE-Vio+770%2C+REAfinity/pmc12495166-412-6-14
    Average 94 stars, based on 53 article reviews
    staining with cd137 - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Enhanced solid tumor cell targeting by a neoepitope-encoding oncolytic measles virus combined with CAR therapy"

    Article Title: Enhanced solid tumor cell targeting by a neoepitope-encoding oncolytic measles virus combined with CAR therapy

    Journal: Molecular Therapy Oncology

    doi: 10.1016/j.omton.2025.201043

    CAR-T cells targeting the MICB-derived octapeptide show CAR-dependent activation and efficiently kill tumor cells (A) Schematic representation of scFv of both 8-VHVL CAR and 8-VLVH CAR targeting the octapeptide VLQSQRTD used in this study. (B) Expression of CAR molecules on T cells. Left panel shows representative plots of flow cytometry data of primary T cells, 8-VHVL CAR-T and 8-VLVH CAR-T. Right panel shows expression of both experimental CARs (8-VHVL and 8-VLVH) as well as CD4 and CD19 CAR on T cells. Black bars represent CAR expression detected via anti-mouse F(abʹ) 2 antibody, and green bars represent EGFP signal; data from n = 18 independent experiments are depicted as mean (SEM). (C) CAR-T cells were cocultured with Mac-1 cells stably transduced to express indicated transgenes. Tumor cells were labeled with CellTrace Violet, and killing was assessed via PI staining. Killing is depicted as ratio of PI+ cells in cocultures and independently cultured Mac-1 cells. Data from n ≥ 6 experiments are depicted as mean (SEM). (D) CD137 expression was measured on CAR-T cells after incubation with Mac-1 for 24 h. Data are shown from EGFP+ CAR-T cells and are depicted from n = 6 independent experiments as mean (SEM). (E) CAR-T cells were cocultured with T cells from the same donor transduced to express indicated transgenes. T cells used as target cells were labeled with CellTrace Violet, and killing was assessed via PI staining. Only EGFP+ target T cells were analyzed for PI staining. Killing is depicted as the ratio of PI+ cells in cocultures and independently cultured target T cells. Data from n = 9 independent experiments are shown as mean (SEM). (F) CD137 expression was measured on CAR-T cells after incubation with transduced T cells as target cells for 24 h. Data are shown from EGFP+ CAR-T cells and are depicted from n = 6 independent experiments as mean (SEM). (G) LAG-3 expression was measured on CAR-T cells after incubation with transduced T cells as target cells for 72 h. Data are shown from EGFP+ CAR-T cells from n = 4 experiments as mean (SEM); statistical analysis of (C) through (G) was performed by two-way ANOVA followed by Dunnett’s multiple-comparisons test, and experimental groups were compared to CD19 CAR. ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001, and ∗∗∗∗ = p ≤ 0.0001.
    Figure Legend Snippet: CAR-T cells targeting the MICB-derived octapeptide show CAR-dependent activation and efficiently kill tumor cells (A) Schematic representation of scFv of both 8-VHVL CAR and 8-VLVH CAR targeting the octapeptide VLQSQRTD used in this study. (B) Expression of CAR molecules on T cells. Left panel shows representative plots of flow cytometry data of primary T cells, 8-VHVL CAR-T and 8-VLVH CAR-T. Right panel shows expression of both experimental CARs (8-VHVL and 8-VLVH) as well as CD4 and CD19 CAR on T cells. Black bars represent CAR expression detected via anti-mouse F(abʹ) 2 antibody, and green bars represent EGFP signal; data from n = 18 independent experiments are depicted as mean (SEM). (C) CAR-T cells were cocultured with Mac-1 cells stably transduced to express indicated transgenes. Tumor cells were labeled with CellTrace Violet, and killing was assessed via PI staining. Killing is depicted as ratio of PI+ cells in cocultures and independently cultured Mac-1 cells. Data from n ≥ 6 experiments are depicted as mean (SEM). (D) CD137 expression was measured on CAR-T cells after incubation with Mac-1 for 24 h. Data are shown from EGFP+ CAR-T cells and are depicted from n = 6 independent experiments as mean (SEM). (E) CAR-T cells were cocultured with T cells from the same donor transduced to express indicated transgenes. T cells used as target cells were labeled with CellTrace Violet, and killing was assessed via PI staining. Only EGFP+ target T cells were analyzed for PI staining. Killing is depicted as the ratio of PI+ cells in cocultures and independently cultured target T cells. Data from n = 9 independent experiments are shown as mean (SEM). (F) CD137 expression was measured on CAR-T cells after incubation with transduced T cells as target cells for 24 h. Data are shown from EGFP+ CAR-T cells and are depicted from n = 6 independent experiments as mean (SEM). (G) LAG-3 expression was measured on CAR-T cells after incubation with transduced T cells as target cells for 72 h. Data are shown from EGFP+ CAR-T cells from n = 4 experiments as mean (SEM); statistical analysis of (C) through (G) was performed by two-way ANOVA followed by Dunnett’s multiple-comparisons test, and experimental groups were compared to CD19 CAR. ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001, and ∗∗∗∗ = p ≤ 0.0001.

    Techniques Used: Derivative Assay, Activation Assay, Expressing, Flow Cytometry, Stable Transfection, Labeling, Staining, Cell Culture, Incubation

    Related Articles

    Incubation:

    Article Title: PGE 2 inhibits TIL expansion by disrupting IL-2 signalling and mitochondrial function
    Article Snippet: .. After overnight incubation, T cells were recovered and the upregulation of CD137 in T cells was evaluated by staining with anti-CD137 (Miltenyi), anti-CD3 (Biolegend or BD Biosciences), anti-CD4 (BD Biosciences), anti-CD8 (BD Biosciences) and anti-mouse TCRβ-constant (Thermo Fisher Scientific) and with Aqua viability dye (Thermo Fisher Scientific). .. With Jurkat cells, the luciferase assay was performed using the Bio-Glo Luciferase Assay System (Promega).

    Article Title: PGE 2 inhibits TIL expansion by disrupting IL-2 signalling and mitochondrial function.
    Article Snippet: .. After overnight incubation, T cells were recovered and the upregulation of CD137 in T cells was evaluated by staining with anti-CD137 (Miltenyi), anti-CD3 (Biolegend or BD Biosciences), anti-CD4 (BD Biosciences), anti-CD8 (BD Biosciences) and anti-mouse TCRβ-constant (Thermo Fisher Scientific) and with Aqua viability dye (Thermo Fisher Scientific). .. With Jurkat cells, the luciferase assay was performed using the Bio-Glo Luciferase Assay System (Promega).

    Article Title: A comprehensive proteogenomic pipeline for neoantigen discovery to advance personalized cancer immunotherapy
    Article Snippet: .. After overnight incubation, T cells were recovered and the upregulation of CD137 was evaluated by staining with anti-CD137 (Miltenyi; dilution of 2:100), anti-CD3 (Biolegend or BD Biosciences; dilution of 1:100), anti-CD4 (BD Biosciences; dilution of 3:100), anti-CD8 (Biolegend; dilution of 1:100) and anti-mouse TCRβ-constant (Thermo Fisher Scientific dilution of 2:100) antibodies and with Aqua viability dye (Thermo Fisher Scientific). .. Flow cytometer LSRFortessaTM (BD Bioscience) or IntelliCyt iQue Screener PLUS (Bucher Biotec) were used for the fluorescence readout and the results were analyzed with FlowJo 10 (TreeStar).

    Staining:

    Article Title: PGE 2 inhibits TIL expansion by disrupting IL-2 signalling and mitochondrial function
    Article Snippet: .. After overnight incubation, T cells were recovered and the upregulation of CD137 in T cells was evaluated by staining with anti-CD137 (Miltenyi), anti-CD3 (Biolegend or BD Biosciences), anti-CD4 (BD Biosciences), anti-CD8 (BD Biosciences) and anti-mouse TCRβ-constant (Thermo Fisher Scientific) and with Aqua viability dye (Thermo Fisher Scientific). .. With Jurkat cells, the luciferase assay was performed using the Bio-Glo Luciferase Assay System (Promega).

    Article Title: PGE 2 inhibits TIL expansion by disrupting IL-2 signalling and mitochondrial function.
    Article Snippet: .. After overnight incubation, T cells were recovered and the upregulation of CD137 in T cells was evaluated by staining with anti-CD137 (Miltenyi), anti-CD3 (Biolegend or BD Biosciences), anti-CD4 (BD Biosciences), anti-CD8 (BD Biosciences) and anti-mouse TCRβ-constant (Thermo Fisher Scientific) and with Aqua viability dye (Thermo Fisher Scientific). .. With Jurkat cells, the luciferase assay was performed using the Bio-Glo Luciferase Assay System (Promega).

    Article Title: A comprehensive proteogenomic pipeline for neoantigen discovery to advance personalized cancer immunotherapy
    Article Snippet: .. After overnight incubation, T cells were recovered and the upregulation of CD137 was evaluated by staining with anti-CD137 (Miltenyi; dilution of 2:100), anti-CD3 (Biolegend or BD Biosciences; dilution of 1:100), anti-CD4 (BD Biosciences; dilution of 3:100), anti-CD8 (Biolegend; dilution of 1:100) and anti-mouse TCRβ-constant (Thermo Fisher Scientific dilution of 2:100) antibodies and with Aqua viability dye (Thermo Fisher Scientific). .. Flow cytometer LSRFortessaTM (BD Bioscience) or IntelliCyt iQue Screener PLUS (Bucher Biotec) were used for the fluorescence readout and the results were analyzed with FlowJo 10 (TreeStar).

    other:

    Article Title: A comprehensive proteogenomic pipeline for neoantigen discovery to advance personalized cancer immunotherapy.
    Article Snippet: Recovered HLA-I and HLA-II peptides were dried using vacuum centrifugation (Concentrator Plus, Eppendorf) and stored at −20 °C.



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    staining with cd137  (Miltenyi Biotec)
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    Miltenyi Biotec staining with cd137
    CAR-T cells targeting the MICB-derived octapeptide show CAR-dependent activation and efficiently kill tumor cells (A) Schematic representation of scFv of both 8-VHVL CAR and 8-VLVH CAR targeting the octapeptide VLQSQRTD used in this study. (B) Expression of CAR molecules on T cells. Left panel shows representative plots of flow cytometry data of primary T cells, 8-VHVL CAR-T and 8-VLVH CAR-T. Right panel shows expression of both experimental CARs (8-VHVL and 8-VLVH) as well as CD4 and CD19 CAR on T cells. Black bars represent CAR expression detected via anti-mouse F(abʹ) 2 antibody, and green bars represent EGFP signal; data from n = 18 independent experiments are depicted as mean (SEM). (C) CAR-T cells were cocultured with Mac-1 cells stably transduced to express indicated transgenes. Tumor cells were labeled with CellTrace Violet, and killing was assessed via PI staining. Killing is depicted as ratio of PI+ cells in cocultures and independently cultured Mac-1 cells. Data from n ≥ 6 experiments are depicted as mean (SEM). (D) <t>CD137</t> expression was measured on CAR-T cells after incubation with Mac-1 for 24 h. Data are shown from EGFP+ CAR-T cells and are depicted from n = 6 independent experiments as mean (SEM). (E) CAR-T cells were cocultured with T cells from the same donor transduced to express indicated transgenes. T cells used as target cells were labeled with CellTrace Violet, and killing was assessed via PI staining. Only EGFP+ target T cells were analyzed for PI staining. Killing is depicted as the ratio of PI+ cells in cocultures and independently cultured target T cells. Data from n = 9 independent experiments are shown as mean (SEM). (F) CD137 expression was measured on CAR-T cells after incubation with transduced T cells as target cells for 24 h. Data are shown from EGFP+ CAR-T cells and are depicted from n = 6 independent experiments as mean (SEM). (G) LAG-3 expression was measured on CAR-T cells after incubation with transduced T cells as target cells for 72 h. Data are shown from EGFP+ CAR-T cells from n = 4 experiments as mean (SEM); statistical analysis of (C) through (G) was performed by two-way ANOVA followed by Dunnett’s multiple-comparisons test, and experimental groups were compared to CD19 CAR. ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001, and ∗∗∗∗ = p ≤ 0.0001.
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    CAR-T cells targeting the MICB-derived octapeptide show CAR-dependent activation and efficiently kill tumor cells (A) Schematic representation of scFv of both 8-VHVL CAR and 8-VLVH CAR targeting the octapeptide VLQSQRTD used in this study. (B) Expression of CAR molecules on T cells. Left panel shows representative plots of flow cytometry data of primary T cells, 8-VHVL CAR-T and 8-VLVH CAR-T. Right panel shows expression of both experimental CARs (8-VHVL and 8-VLVH) as well as CD4 and CD19 CAR on T cells. Black bars represent CAR expression detected via anti-mouse F(abʹ) 2 antibody, and green bars represent EGFP signal; data from n = 18 independent experiments are depicted as mean (SEM). (C) CAR-T cells were cocultured with Mac-1 cells stably transduced to express indicated transgenes. Tumor cells were labeled with CellTrace Violet, and killing was assessed via PI staining. Killing is depicted as ratio of PI+ cells in cocultures and independently cultured Mac-1 cells. Data from n ≥ 6 experiments are depicted as mean (SEM). (D) <t>CD137</t> expression was measured on CAR-T cells after incubation with Mac-1 for 24 h. Data are shown from EGFP+ CAR-T cells and are depicted from n = 6 independent experiments as mean (SEM). (E) CAR-T cells were cocultured with T cells from the same donor transduced to express indicated transgenes. T cells used as target cells were labeled with CellTrace Violet, and killing was assessed via PI staining. Only EGFP+ target T cells were analyzed for PI staining. Killing is depicted as the ratio of PI+ cells in cocultures and independently cultured target T cells. Data from n = 9 independent experiments are shown as mean (SEM). (F) CD137 expression was measured on CAR-T cells after incubation with transduced T cells as target cells for 24 h. Data are shown from EGFP+ CAR-T cells and are depicted from n = 6 independent experiments as mean (SEM). (G) LAG-3 expression was measured on CAR-T cells after incubation with transduced T cells as target cells for 72 h. Data are shown from EGFP+ CAR-T cells from n = 4 experiments as mean (SEM); statistical analysis of (C) through (G) was performed by two-way ANOVA followed by Dunnett’s multiple-comparisons test, and experimental groups were compared to CD19 CAR. ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001, and ∗∗∗∗ = p ≤ 0.0001.
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    CAR-T cells targeting the MICB-derived octapeptide show CAR-dependent activation and efficiently kill tumor cells (A) Schematic representation of scFv of both 8-VHVL CAR and 8-VLVH CAR targeting the octapeptide VLQSQRTD used in this study. (B) Expression of CAR molecules on T cells. Left panel shows representative plots of flow cytometry data of primary T cells, 8-VHVL CAR-T and 8-VLVH CAR-T. Right panel shows expression of both experimental CARs (8-VHVL and 8-VLVH) as well as CD4 and CD19 CAR on T cells. Black bars represent CAR expression detected via anti-mouse F(abʹ) 2 antibody, and green bars represent EGFP signal; data from n = 18 independent experiments are depicted as mean (SEM). (C) CAR-T cells were cocultured with Mac-1 cells stably transduced to express indicated transgenes. Tumor cells were labeled with CellTrace Violet, and killing was assessed via PI staining. Killing is depicted as ratio of PI+ cells in cocultures and independently cultured Mac-1 cells. Data from n ≥ 6 experiments are depicted as mean (SEM). (D) CD137 expression was measured on CAR-T cells after incubation with Mac-1 for 24 h. Data are shown from EGFP+ CAR-T cells and are depicted from n = 6 independent experiments as mean (SEM). (E) CAR-T cells were cocultured with T cells from the same donor transduced to express indicated transgenes. T cells used as target cells were labeled with CellTrace Violet, and killing was assessed via PI staining. Only EGFP+ target T cells were analyzed for PI staining. Killing is depicted as the ratio of PI+ cells in cocultures and independently cultured target T cells. Data from n = 9 independent experiments are shown as mean (SEM). (F) CD137 expression was measured on CAR-T cells after incubation with transduced T cells as target cells for 24 h. Data are shown from EGFP+ CAR-T cells and are depicted from n = 6 independent experiments as mean (SEM). (G) LAG-3 expression was measured on CAR-T cells after incubation with transduced T cells as target cells for 72 h. Data are shown from EGFP+ CAR-T cells from n = 4 experiments as mean (SEM); statistical analysis of (C) through (G) was performed by two-way ANOVA followed by Dunnett’s multiple-comparisons test, and experimental groups were compared to CD19 CAR. ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001, and ∗∗∗∗ = p ≤ 0.0001.

    Journal: Molecular Therapy Oncology

    Article Title: Enhanced solid tumor cell targeting by a neoepitope-encoding oncolytic measles virus combined with CAR therapy

    doi: 10.1016/j.omton.2025.201043

    Figure Lengend Snippet: CAR-T cells targeting the MICB-derived octapeptide show CAR-dependent activation and efficiently kill tumor cells (A) Schematic representation of scFv of both 8-VHVL CAR and 8-VLVH CAR targeting the octapeptide VLQSQRTD used in this study. (B) Expression of CAR molecules on T cells. Left panel shows representative plots of flow cytometry data of primary T cells, 8-VHVL CAR-T and 8-VLVH CAR-T. Right panel shows expression of both experimental CARs (8-VHVL and 8-VLVH) as well as CD4 and CD19 CAR on T cells. Black bars represent CAR expression detected via anti-mouse F(abʹ) 2 antibody, and green bars represent EGFP signal; data from n = 18 independent experiments are depicted as mean (SEM). (C) CAR-T cells were cocultured with Mac-1 cells stably transduced to express indicated transgenes. Tumor cells were labeled with CellTrace Violet, and killing was assessed via PI staining. Killing is depicted as ratio of PI+ cells in cocultures and independently cultured Mac-1 cells. Data from n ≥ 6 experiments are depicted as mean (SEM). (D) CD137 expression was measured on CAR-T cells after incubation with Mac-1 for 24 h. Data are shown from EGFP+ CAR-T cells and are depicted from n = 6 independent experiments as mean (SEM). (E) CAR-T cells were cocultured with T cells from the same donor transduced to express indicated transgenes. T cells used as target cells were labeled with CellTrace Violet, and killing was assessed via PI staining. Only EGFP+ target T cells were analyzed for PI staining. Killing is depicted as the ratio of PI+ cells in cocultures and independently cultured target T cells. Data from n = 9 independent experiments are shown as mean (SEM). (F) CD137 expression was measured on CAR-T cells after incubation with transduced T cells as target cells for 24 h. Data are shown from EGFP+ CAR-T cells and are depicted from n = 6 independent experiments as mean (SEM). (G) LAG-3 expression was measured on CAR-T cells after incubation with transduced T cells as target cells for 72 h. Data are shown from EGFP+ CAR-T cells from n = 4 experiments as mean (SEM); statistical analysis of (C) through (G) was performed by two-way ANOVA followed by Dunnett’s multiple-comparisons test, and experimental groups were compared to CD19 CAR. ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001, and ∗∗∗∗ = p ≤ 0.0001.

    Article Snippet: For evaluation of CAR-T cell activation, staining with CD137 (CD137 Antibody, PE-Vio770, anti-human, REAfinity) (Miltenyi Biotech, Bergisch Gladbach, Germany) was carried out after 24 h of co-incubation.

    Techniques: Derivative Assay, Activation Assay, Expressing, Flow Cytometry, Stable Transfection, Labeling, Staining, Cell Culture, Incubation